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Biotech Trading Partners |
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QnE Hyaluronic Acid ELISA Assay
Catalog# BTP-96200
For Research Use Only Multi-species: can be used on human and animal biological fluids
The QnE Hyaluronic Acid (HA) test kit is an enzyme-linked binding protein assay that uses a capture molecule known as hyaluronic acid binding protein (HABP). Properly diluted specimens and HA reference solutions are incubated in HABP-coated microwells (60 minutes at room temperature), allowing HA present in the specimens to react with the immobilized binding protein (HABP). After the removal of unbound molecules by washing, HABP conjugated with horseradish peroxidase (HRP) solution is added to the microwells to form complexes with bound HA (incubated for 30 minutes at room temperature). Following another washing step, a chromogenic substrate of tetramethylbenzidine and hydrogen peroxide is added to develop a colored reaction (incubated for 30 minutes at room temperature). The intensity of the color is measured in optical density (O.D.) units with a spectrophotometer at 450nm. HA levels in specimen and control samples are determined against a reference curve prepared from the reagent blank (0 ng/mL) and the HA reference solutions provided with the kit (50, 100, 200, 500, 800 ng/mL).
· Marker for inflammation in peritoneal dialysis patients · Marker for hepatotoxicity · Toxicological and efficacy studies in drug development · Basic research on liver sinusoidal endothelial cell function · Measuring HA production in cell cultures: yeast, tumor cells, bacterial, various tissue cultures · Development of nutritional supplements · Cosmetic/skin care product development · Not Species Specific |