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INTERPRETATION OF RESULTS

SMART & PRIMATE-ONE ASSAYS

 

Each Control, Calibrator, and Specimen has an OD result from the Antigen Well and the Control Well. The OD of the Control Well is subtracted from the OD of the Antigen Well to yield the Differential OD.

 

For Example:      

 

Antigen Well OD

Control Well OD

Differential OD

Reactive Control

1.82

0.03

1.79

Negative Control

0.12

0.04

0.08

Calibrator

0.38

0.10

0.28

Specimen 1

1.10

0.19

0.91

Specimen 2

0.25

0.02

0.23

 

 

 

The Index Value of the controls and specimens is obtained by dividing the Differential OD of the specimen or control by the Differential OD of the calibrator.

 

Calculation of the Example:       

Differential OD of the Calibrator

0.28

 

 

Index Value of Reactive Control

1.79/0.28

6.39 Index    

Valid

Index Value of Negative Control

0.08/0.28

0.29 Index

Valid

Index Value of Specimen 1

0.91/0.28

3.25 Index    

Positive

Index Value of Specimen 2

0.23/0.28

0.82 Index

Negative

 

An Index Value of 1.0 or greater is considered Positive.

An Index Value of less than 1.0 is considered Negative.

 

Negative Index Values are considered to be 0.00

 

 

 

Biotech Trading Partners will provide software, free of charge to our customers, which will automatically perform your calculations. You just input your OD values from your reader into an excel spreadsheet and the software does the rest.

 

 

Quality Control

The use of controls allows validation of the test. The results should not be used if a control, or the calibrator, is out of range. A run is valid if all three of the following conditions are met:

      Negative Control -     Index Value of less than 0.60

      Reactive Control -     Index Value between 1.50 and 6.50

      Calibrator -        The Differential OD must be greater than 0.00