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Biotech Trading Partners |
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QnE HIV-1 Integrase Assay Kit Catalog# BTP-1700
Introduction
The QnE HIV-1 Integrase Assay Kit is used to quantitatively measure the effects of interacting proteins and other test articles on HIV-1 integrase activity. Streptavidin-coated 96-well plates are coated with a double-stranded HIV-1 LTR U5 donor substrate (DS) oligonucleotide containing an end-labeled biotin. Full-length recombinant HIV-1 integrase protein (200 nM, purified from bacteria) is then loaded onto this oligo substrate. Test articles are added to the reaction and then a different double-stranded target substrate (TS) oligo containing 3’-end modifications is added to the plate. The HIV-1 integrase cleaves the terminal two bases from the exposed 3’-end of the HIV-1 LTR DS and then catalyzes a strand-transfer reaction to integrate the DS into the TS. The products of the reaction are detected colorimetrically using an HRP-labeled antibody directed against the TS 3’-end modification. Sodium azide is included in the kit as a positive control compound that inhibits HIV-1 integrase activity. |