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Biotech Trading Partners |
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QnE-Telomerase Detection Kit Catalog# BTP-400 100 X 25 μl Reactions
The ends of linear human chromosomes terminate with telomers that consist of a tandem repeat of the sequence TTAGGG. The telomerase enzyme is a ribonucleoprotein that synthesizes these telomeric DNA repeat sequences. Telomerase activity is low in healthy cells and increased in a variety of malignancies and in immortalized cells.
The QnE-Telomerase kit is an optimized and validated real-time PCR Kit for the quantification of human telomerase activity that can be utilized in most real-time PCR platforms. In this telomeric repeat amplification protocol (TRAP), the telomerase activity in cell extracts adds the telomeric (TTAGGG) repeat sequences to the 3’-end of an oligonucleotide substrate. The number of repeat sequences added by the enzyme is quantified using real-time PCR by measuring the increase in SYBR green fluorescence upon binding to DNA. Real-time PCR is conducted under conditions in which primer dimer formation is negligible and the extent of repeat amplification is directly proportional to telomerase activity. |