|
Biotech Trading Partners |
|
QnE-DNA Kit
Catalog# BTP-300 2 X 96 Purifications
The QnE-DNA Kit is designed for the high-throughput purification of genomic DNA from cultured cells in a 96-well plate format. No phenol-chloroform extraction or precipitation steps are involved. The kit can also be used to cleanup up to 96 DNA samples at a time. The sample addition and washing steps are performed using any 96-well compatible vacuum manifold, while the final elution of the DNA product is performed using a table-top centrifuge.
Cells are lysed using an SDS-containing buffer and are treated with RNases and proteases, which are then heat-inactivated. A buffer containing the denaturant guanidine isothiocyanate and ethanol are then added to the samples, which are then added to the filter plates. This step facilitates the binding of DNA to the filter plate’s silica membrane matrix. Under these conditions the DNA binds to the membrane while other contaminants are washed through. The QnE-DNA Kit 96-well silica membrane plates have a standard Society for Biomolecular Screening (SBS) footprint and are compatible with a variety of automated liquid handling workstations, all 96-well-compatible vacuum manifolds and most, if not all, 96-well plate-compatible rotors. The plate is then washed to further remove protein, buffer components and other contaminants using two ethanol-containing wash buffers and the final genomic DNA product is eluted in TE. The final DNA product is essentially free of RNA and can be used directly for quantitative PCR and other downstream applications. Very small fragments of DNA including primers (<200 nucleotides) are not efficiently isolated using this kit. |